Protein Resins
AdipoGen ST2 humanFc human rec
Protein. Signal peptide and the sequence of isoform 2 of human ST2 aa 1-328 are fused at the C-terminus to the Fc portion of human IgG1. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in PBS, pH 7.2. Binds to human IL-33. Purity >90% SDS-PAGE. The ST2 Interleukin-1 receptor-like 1 Interleukin-33 receptor gene was originally identified as a gene induced by serum or oncogene expression in fibroblasts. The gene produces a shorter soluble secreted form ST2 and a longer, transmembrane form ST2L by alternative splicing. Soluble ST2 has been shown to downregulate the expression of TLR1 and TLR4. ST2L negatively regulates TLR4 signaling and induces endotoxin tolerance, and enhances Th2 responses. IL-33 is the specific ligand for ST2L.
Wl Gore And Associates 3.5 mL GORE Protein Capture Device with Protein A for Rapid mAb Purification
GORE Protein Capture Devices with immobilized Protein A are intended for small-scale affinity purification of monoclonal antibodies and other proteins demonstrating an Fc region. GORE Protein Capture Devices use a unique expanded polytetrafluoroethylene (ePTFE) membrane composite that provides a bindding capacity advantage at high flow rates and improved the speed of purification. In addition the GORE Protein Capture Deivces produce highly concentrated elution pools, which may eliminate a downstream concentration step during the antibody purification process. These unique membrane devices are offered in 1 mL (≥ 30 mg capacity), 3.5 mL (≥ 105 mg capacity) and 9.0 mL (≥27 mg capacity) at 20 seconds residence time.
Sigma Aldrich Fine Chemicals Biosciences Gammabind Plus SepharoseR
GammaBind Plus Sepharose(R) is used for lab-scale affinity purification of IgG from a large variety of species and has better binding to rat and mouse IgG subclasses compared to GammaBind Sepharose.GammaBind Plus Sepharose(R) is GammaBind G Type 3 covalently immobilized to Sepharose(R) CL-6B by malimide linkage. GammaBind G Type 3 a recombinant form of streptococcal protein G binds to the Fc region of IgG from a variety of mammalian species. GammaBind Plus Sepharose(R) may be used to analyze and purify classes subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium. Since only the Fc region is involved in binding the Fab region is still available for binding antigen.
Genscript Corporation Protein G Resin
GenScript Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is over 20 mg sheep IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Sigma Aldrich Fine Chemicals Biosciences MabSelectTM PrismA Cytiva
MabSelect PrismA affinity chromatography resin has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein A-derived ligand. This allows future demands in mAb purification to be met including processing of many bispecific antibodies.Enhanced dynamic binding capacity allows high mass throughput of processed mAb per resin volume unit.Excellent alkaline stability enables efficient cleaning and sanitization using 0.5-1.0 M NaOH for improved process economy bioburden control and robustness.The enhanced alkaline stability of MabSelect PrismA also provides opportunities for more robust mAb purification using periodic counter-current chromatography. The possibility for cleaning using 1 M NaOH increases bioburden control for continuous processes which can be exposed to days or weeks of feed harvest.
Sigma Aldrich Fine Chemicals Biosciences HiTrapR MabSelectTM PrismA
These HiTrap columns are prepacked with MabSelect PrismA protein A chromatography resin. This affinity resin in the packed column has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein ligand allowing efficient cleaning between monoclonal antibody purification runs. This allows future demands in monoclonal antibody purification to be met including processing of many bispecific antibodies.Enhanced dynamic binding capacity compared with other protein A resins.Excellent alkaline stability enables efficient cleaning and sanitization using 0.5-1.0 M NaOH.Convenient HiTrap format for easy connection to a syringe peristaltic pump or chromatography systems such as an A„KTA system for convenient process optimization.The enhanced alkaline stability of MabSelect PrismA also provides opportunities for more robust mAb purification using periodic counter-current chromatography. The possibility for cleaning using 1 M NaOH increases bi
Biotang Inc Protein A/G Recombinant, Protein A/G is an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from these other serum proteins.
Protein A/G Recombinant, Protein A/G is an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from these other serum proteins.
Sigma Aldrich Fine Chemicals Biosciences MabSelect SuReTM LX Cytiva
MabSelect(TM) SuRe(TM) LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes. Alkali tolerance high capacity and low ligand leakage in combination with the rigid base matric makes MabSelect(TM) SuRe(TM) LX highly suitable for purification of monoclonal anitbodies for clinical applications.MabSelect(TM) SuRe(TM) LX is a member of the MabSelect(TM) family of affinity chromatography media for the capture of monoclonal antibodies (MAbs) at process scale. MabSelect(TM) SuRe(TM) LX shares features and benefits of MabSelect(TM) and MabSelect(TM) SuRe(TM) such as a rigid high-flow agarose matrix that ensures excellent pressure/flow properties low non-specific binding that leads to low impurity levels in the eluate pool and an oriented ligand coupling for optimal binding capacity. It also features the same alkali-tolerant rProtein A ligand as MabSelect(TM) SuRe. This ligand provides greater st